Effect of pH on the structure and stability of irisin, a multifunctional protein: Multispectroscopic and molecular dynamics simulation approach

Irisin is a potential therapeutic agent to prevent or treat various metabolic-related disorders and neurodegenerative diseases viz. Alzheimer's disease (AD). In this study, we have employed multispectroscopic approach elucidate the structural conformational changes in irisin at varying pH (pH 2.0 12.0). The spectroscopic measurements revealed that maintains its structure (both secondary tertiary) alkaline range, with minimal observed across it. However, tertiary alterations were evident acidic range. CD spectroscopy suggested gain of implying more stable pH, maximum stability compactness 4.0. vitro observations further validated by silico studies. Molecular dynamics simulation also assumes higher space 4.0 6.0 than rest system. This study can serve as platform delineate enhanced functionality lower be implicated developing therapies for metabolic disorders, including diabetes obesity.